This edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. Furthermore, it is especially well suited for larger complexes and molecular machines, although it can also be used for macromolecules as small as 4...

Pirates are still out there, flintlock pistols and schooners traded for assault rifles and black-painted Zodiacs. PARAISO, Book One of Pirates, Scoundrels, and Saints tells the riveting tale of modern-day pirates in the unpredictable waters of the Caribbean—not Jack Sparrow caricatures, but real men and women driven by their pasts. Juan is fiercely loyal to his crew and loves adventure. His son Ivan longs for a respectable life and to win the beautiful daughter of a prominent Dominican Rep...