Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. - Prepared by experts in the field - Provides proven labo...

This book comprehensively covers the mechanisms of action and inhibitor design for HIV-1 integrase. It serves as a resource for scientists facing challenging drug design issues and researchers in antiviral drug discovery. Despite numerous review articles and isolated book chapters dealing with HIV-1 integrase, there has not been a single source for those working to devise anti-AIDS drugs against this promising target. But this book fills that gap and offers a valuable introduction to the f...

Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages.Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems...

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this st...

This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinforma...

This highly illustrated book provides an up-to-date description of the structure and function of the translation system including ribosomes, tRNAs, translation factors, antibiotics and aminoacyl-tRNA synthetases.Research on translation is undergoing rapid changes and is receiving significant attention as evidenced by the Nobel Prize in Chemistry 2009. The structural research by crystallography and cryo-EM forms part of an interactive framework that involves biochemistry and molecular compu...

Molecular Biology: Academic Cell Update Edition Study Guide aims to help students get a better grasp of important concepts in Molecular Biology: Academic Cell Update. Each of the chapters in this study guide contains a summary that lets students see the bigger picture, that is, how all the techniques fit together in that chapter. A list of terms is presented in a concise format rather than spread throughout the textbook chapter to help the student remember these key terms in preparation fo...

The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather...

This book provides comprehensive up-to-date information on the structure and function of immunoglobulins. It describes the basic features of these molecules, which assists the reader in understanding how they function as an integral part of the immune system. The Immunoglobulins describes the localization and structure of different binding sites of immunoglobulin molecules, including the antigen-binding site, on the basis of latest x-ray crystallography studies. It discusses recently devel...

A reference on cellular signaling processes, the third edition of Signal Transduction continues in the tradition of previous editions, in providing a historical overview of how the concept of stimulus-response coupling arose in the early twentieth century and shaped our current understanding of the action of hormones, cytokines, neurotransmitters, growth factors and adhesion molecules. In a new chapter, an introduction to signal transduction, the book provides a concise overview of recepto...

Standard medicinal chemistry courses and texts are organized by classes of drugs with an emphasis on descriptions of their biological and pharmacological effects. This book represents a new approach based on physical organic chemical principles and reaction mechanisms that allow the reader to extrapolate to many related classes of drug molecules. The Second Edition reflects the significant changes in the drug industry over the past decade, and includes chapter problems and other elements t...

Epigenetics is the study of heritable changes in gene expression or cellular phenotype, caused by mechanisms other than changes in the DNA sequence. Examples include DNA methylation and histone modification. These changes may remain through cell divisions and multiple generations. Epigenetic differences among individuals may account for some of the differences between monozygotic (identical) twins. Aberrant DNA methylation is also frequently associated with human aging and diseases, such a...